Microbicidal Potentialities of Essential Oil from Pogostemon benghalensis and P. cablin

A comprehensive study on the antimicrobial properties of essential oils extracted from medicinal plants

Introduction

Essential oils derived from aromatic plants have gained significant attention in recent years due to their potential antimicrobial properties. Among these, Pogostemon benghalensis and Pogostemon cablin have been traditionally used in various medicinal applications .

Pogostemon benghalensis

A perennial herb native to Southeast Asia, traditionally used for its medicinal properties in treating various ailments.

Pogostemon cablin

Commonly known as patchouli, this plant is widely cultivated for its essential oil used in perfumery and traditional medicine.

Research Objective: This study aims to evaluate the microbicidal potential of essential oils extracted from P. benghalensis and P. cablin against various pathogenic microorganisms .

Methodology

The essential oils were extracted through hydrodistillation using a Clevenger-type apparatus. The chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS) .

Tested Microorganisms
  • Gram-positive bacteria: Staphylococcus aureus, Bacillus subtilis
  • Gram-negative bacteria: Escherichia coli, Pseudomonas aeruginosa
  • Fungi: Candida albicans, Aspergillus niger
Extraction Method

Hydrodistillation using Clevenger apparatus for 3 hours

Experimental Design Overview
Plant Collection
Oil Extraction
GC-MS Analysis
Antimicrobial Tests

Results

The essential oils exhibited significant antimicrobial activity against the tested microorganisms. P. cablin showed stronger inhibitory effects compared to P. benghalensis .

P. benghalensis Results
P. cablin Results
Minimum Inhibitory Concentration (MIC) Values
Microorganism P. benghalensis MIC (μL/mL) P. cablin MIC (μL/mL)
Staphylococcus aureus 12.5 6.25
Escherichia coli 25.0 12.5
Candida albicans 6.25 3.12
Aspergillus niger 12.5 6.25

Discussion

The results demonstrate that both essential oils possess significant antimicrobial properties, with P. cablin exhibiting superior activity. This could be attributed to its higher content of patchoulol and other bioactive compounds .

Potency Comparison

P. cablin showed approximately 2-fold higher antimicrobial activity compared to P. benghalensis .

Chemical Composition

GC-MS analysis revealed the presence of sesquiterpenes as major constituents in both oils .

Mechanism of Action

The oils likely disrupt microbial cell membranes, leading to cell death .

Key Finding

The essential oils showed broad-spectrum antimicrobial activity, with particularly strong effects against fungal strains, suggesting potential applications in antifungal formulations .

Conclusion

This study confirms the significant microbicidal potential of essential oils from Pogostemon benghalensis and P. cablin. The results support their traditional use in medicinal applications and suggest potential for development as natural antimicrobial agents .

Future Research Directions
  • Investigation of synergistic effects with conventional antibiotics
  • In vivo studies to confirm efficacy and safety
  • Development of formulations for practical applications
  • Exploration of other biological activities